Izindlela

1. Indlela ye-Bioburden: Ukuqinisekisa Ukufa

• ? Ukubala Okuqondile/Indlela Yokubala
• ? Indlela Yengxenyana-Negative

2. Indlela Ye-Overkill: Ukubonisa ?12-Log Ukuncishiswa

• ? Indlela Yomjikelezo Wesigamu
• ? Indlela Yokuthuthukisa Umjikelezo

Imigomo Ebalulekile

• I-Bioburden
• 12-log ukunciphisa
• I-D-value

Uhlolojikelele lwezindlela

1. Indlela Yomjikelezo Wohhafu (Isimiso Esiyisisekelo – Ukubala Kokugijima)

• I-bioburden yokuqala yethiyori: 10?
• Imibandela Yokuhlola:
  • 1 ukugijima nabasindile kuvunyelwe (“ingxenye yomjikelezo” noma “umjikelezo omfushane”)
  • Imijikelezo enguhhafu engu-3 enabasindile abaziro abadingekayo

2. Indlela Yokubalwa Komjikelezo

• Idingai-bioburden yokuqala eyaziwayo & izinga lokufa
• Isisekelo sokukhetha indlela: BhekaIndlela Yengxenyana-Negative

3. Indlela yokubala Okuqondile

• Idingai-bioburden yokuqala eyaziwayo
• Imibandela Yokuhlola:
  • 1 gijima ngaphandle kwe-sterilant (control)
  • 1 run ekhombisa ?4-log ukunciphisa
  • 3 igijima namazinga okunciphisa amaphakathi

4. Indlela Yengxenyana-Negative

• Idingai-bioburden yokuqala eyaziwayo
• Amamodeli Ezibalo:
  • A3.2 HSKP (Inqubo ye-Holcomb-Spearman-Karber)
  • A3.3 SMCP (Inqubo ye-Stumbo-Murphy-Cochran)

Izinhlobo Zezinkomba Zebhayoloji (BIs)

• I-Strip/Disk BI
  I-Strip/Disk BIInkampani yenethiwekhi ene-microorganism-coated (isb., iphepha/idiski lesihlungi) edinga ukudluliselwa kumidiya yokukhula ukuze ifukamele.
• I-Ampoule-based BI
  I-ampoule yengilazi equkethe izinhlamvu kanye nendawo yokukhula ehlukene (idinga ukuchotshozwa okwenziwa ngesandla ukuze isebenze).
• 
  I-BI Ezimele
  Iyunithi ehlanganisiwe: I-Spore strip + ukukhula okuphakathi ku-vial eyodwa (ukwenziwa kusebenze ngokuminyanisa).

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