Cov txheej txheem

1. Bioburden Method: Kev lees paub kev tuag

• ? Direct Count/Enumeration Method
• ? Fraction-Negative Method

2. Txoj Kev Tshaj Tawm: Ua kom pom ?12-Kev txo qis

• ? Ib nrab-Cycle Method
• ? Cycle Development Method

Cov ntsiab lus tseem ceeb

• Bioburden
• 12-log txo
• D-tus nqi

Txheej txheem txheej txheem

1. Half-Cycle Method (Lub Ntsiab Cai – Khiav suav)

• Theoretical pib bioburden: 10?
• Kev Xeem Xeem:
  • 1 khiav nrog cov muaj sia nyob tso cai (“ib nrab lub voj voog” los yog “luv luv”)
  • 3 ib nrab-lub voj voog nrog xoom survivors xav tau

2. Txoj kev xam lub voj voog

• Yuav tsum muajpaub qhov pib bioburden & tus nqi tuag
• Txoj kev xaiv hauv paus: SaibFraction-Negative Method

3. Direct Count Method

• Yuav tsum muajpaub qhov pib bioburden
• Kev Xeem Xeem:
  • 1 khiav tsis muaj sterilant (tswj)
  • 1 khiav ua qauv qhia ?4-log txo
  • 3 khiav nrog kev txo qis nruab nrab

4. Fraction-Negative Method

• Yuav tsum muajpaub qhov pib bioburden
• Statistical Models:
  • A3.2 HSKP (Holcomb-Spearman-Karber txheej txheem)
  • A3.3 SMCP (Stumbo-Murphy-Cochran txheej txheem)

Hom Kev Ntsuas Kab Mob (BIs)

• Strip/Disk BI
  Strip/Disk BIMicroorganism-coated carrier (piv txwv li, ntawv / lim disk) yuav tsum tau hloov mus rau kev loj hlob media rau incubation.
• Ampoule-Based BI
  iav ampoule uas muaj spores thiab cais kev loj hlob nruab nrab (yuav tsum tau phau ntawv crushing kom qhib).
• 
  Self-Contained BI
  Integrated unit: Spore strip + kev loj hlob nruab nrab hauv ib lub vial (ua kom los ntawm compression).

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